作者單位：鹹寧學院藥學院藥理教研室;鹹寧學院藥學院護理系;鹹寧學院藥學院藥理教研室;鹹寧學院藥學院免疫教研室;鹹寧學院藥學院生化教研室 鹹寧437100;湖北;鹹寧437100;湖北;鹹寧437100;湖北;鹹寧437100;湖北;鹹寧437100;湖北
關鍵詞：竹葉;抗氧化;超氧化物歧化酶;氧自由基;羥自由基
基金：湖北省教育廳科研基金資助項目 (№ 2 0 0 3A0 0 8)
分類號：R285.5
DOI：cnki:ISSN:1009-2501.0.2004-05-025
正文快照：
竹葉是禾木科多年生常綠喬木或灌木植物淡竹的葉片,在我國具有悠久的食用和藥用歷史。竹葉性淡、微澀、寒,味甘、苦。近年來對竹葉提取物藥用價值和保健功能的研究才剛起步,研究表明,竹葉中的總黃酮含量與銀杏葉具有可比性,竹葉還含有大量的活性多糖成分和豐富的微量元素,具有
AIM: To study the antioxidant effects of extract solution of bamboo leaves in mice and the effects of scavenging hydroxyl free radical ( ·OH- ) in vitro. METHODS: The mice were treated with extract solution of bamboo leaves (8,12 g·kg -1 ,ig) daily for 30 d. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in blood and the contents of lipofuscin (LF),malondialdehyde (MDA) and SOD in liver,and brain and heart by biochemical methods were determined. ·OH- was induced by ascorbate-Cu 2+ reaction system,and the effect of extract solution of bamboo leaves on scavenging ·OH- was estimated by colorimetry. RESULTS: The extract solution of bamboo leaves could significantly increase the activities of SOD and GSH-Px in blood and the activities of SOD in liver,brain and heart in mice,and obviously reduce the contents of LF and MDA in mice tissues. The extract solution of bamboo leaves of 0.05 - 1.0 g·L -1 had markedly effects on scavenging ·OH- from 7.8 % to 87.7 % in dose-effect relationship (r= 0.976 ). CONCULSION: The extract solution of bamboo leaves can obviously inhibit damage of free radical and prevent lipid peroxidation. The mechanisms may associate with directly scavenging ·OH- ,and with increasing the activities of antioxidation enzymes in mice tissues.